Comparison of two PCRs for detection of Mycobacterium ulcerans.
نویسندگان
چکیده
Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.
منابع مشابه
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 37 1 شماره
صفحات -
تاریخ انتشار 1999